Journal: Cell
Article Title: Hypoxia rescues frataxin loss by restoring iron sulfur cluster biogenesis
doi: 10.1016/j.cell.2019.03.045
Figure Lengend Snippet: (A) Immunoblot from brain and heart of WT and shFXN mice housed in 21% O2 or 11% O2 at week 12, blotted for FXN and Actin. (B) Hematocrit measurements from WT and shFXN mice housed in 21% O2 or 11% O2 at week 8 (n≥ 3 per group). (C) Inverted screen test analysis for WT or shFXN mice housed in 21% O2 or 11% O2 at 12 weeks. Time to fall is represented (n≥ 7 per group). (D) Accelerating rotarod analysis for WT or shFXN KD mice housed in 21% O2 or 11% O2 at 12 weeks. Latency to fall measured as mean value of triplicate trials per mouse (n≥ 7 per group). (E) Representative images of Purkinje cell layer stained with parvalbumin, PCP-2 and Hoechst 33342 from WT or shFXN mice housed in 21% O2 or 11% O2. Scale bar = 30μm. (F) Inverted screen test analysis for WT or shFXN mice housed in 21% O2 or 55% O2 at 6 weeks. Time to fall is represented (n≥ 5 per group). (G) Accelerating rotarod analysis for WT or shFXN mice housed in 21% O2 or 55% O2 at 6 weeks. Latency to fall measured as mean value of triplicate trials per mouse (n≥ 5 per group). (H) Survival of WT or FXN knockdown (shFXN) mice housed in 21% O2 or 11% O2 (n=10 per group). (I) Quantification of corrected QT interval, as observed by ECG, of WT or shFXN mice housed in 21% O2 or 11% O2 at 12 weeks (n≥ 3 per group). All bar plots show mean ± SD. *=p < 0.05, **=p < 0.01, ***=p < 0.001, ****=p < 0.0001. One-way ANOVA with Bonferroni’s post-test. See also Figure S7.
Article Snippet: Using subcutaneous three-lead ECG needle electrodes (Bio Amp, ADInstruments, Dunedin, New Zealand), ECG signal was recorded at 4 kHz and filtered using a high- and low- pass settings of 0.3 Hz and 1 kHz respectively (PowerLab, Dunedin, New Zealand).
Techniques: Western Blot, Staining, Knockdown
